The research in my laboratory is aimed at understanding and utilizing adenovirus as a vector for anti-angiogensis of cancer therapy.
    Human adenoviruses are molecular parasites that rely on cellular mechanisms for expression of their reporter gene in formation. Recombination of foreign genes like endostation, angostetion, neurotrophin in to adenovirus can express efficiently with eukyrotic in target tissues or organs:
    The adenovirus infections cycle can be described in terms of an orderly program of expression of viral genes that comprise the double-stranded DNA genome, Culmination the synthesis of prodigious quantities of viral macromolecule and in limitation of cellular expression. The viral depends on the cellular biosynthetic machinery, yet induces transnational and post-transcription regulatory mechanisms control viral and gene expression in infected cells. The inhibition of cellular gene expression characteristic of the late phase of adenovirus infection is in part the result of an unusual post-transcriptional regulatory mechanic induction of selective export of newly-synthesized viral mRNAs from the nucleics to cytoplasm. Two viral early proteins, the EIB55kDa, E4 Orf 6 protein and tripatite leader sequence of major late promoter, are for efficient export of viral mRNAs with concomitant inhibition of export of the most newly synthesized cellular mRNA species. Our work aims to elucidate such regulatory events (Ref. J. Viral 1998, 72(1):225-235)
    The early E2E promoter, which controls production of viral replication proteins, was extensively characterized in early studies. More recently, we have established that typical RNA polymerase ¢é8òé0 promoter is also transcribed by RNA polymerase ¢é8óé( in adenoviruses infected cells. The properties of RNA polymerase ¢é8óé( transcription, suggest that recognition of the E2E promoter of polymerase ¢é8óé( may serve to switch initiator, or ignite a threshold for RNA polymerase ¢é8òé0 transcription.(Ref. J. of Virology, 77(7:4015-4024))
    Those novel regulatory mechanisms would ensure that production of replication protein and therefore entry into late phase of viral infection, take place only when the host cell has been optimized for viral replication by the action of E1A proteins that both entry of infected cells into S phase and efficient RNA polymerase ¢é8òé0 transcription the E2E promoter. There is the first time to demonstrate by experiment which promoter in adenovirus can simultaneously utilizing RNA polymerase ¢é8òé0 and polymerase ¢é8óé( (Ref, PNAS, 1994,91(4):1265-9).
    HPV types 16 and 18 are the mayor cause of cervical cancer. The early gene E6 and E7 of HPV can effectively bind to P53 and Retinoblastoma respectively during the viral infection. These physical binding from both viral early gene and tumor suppress gene induces cell-cycle arrest or apoptosis. These HPV E6 and E7 protein also regulate by HPV E2 and gene for down regulating of oncogenes during HPV infection. Peptide-nucleic acid is applied for interfering HPV18 E6 and E17 expression to prevent the immobilization of HPV infected cells. (Ref. Infectious 2000,31-1,320-220).

PART OF PUBLICATIONS:
Xiao,Lin...Huang, W. GATA-3 transfusion in T Cell, Foreign Medical Sciences Blood Transfusion and Hematology,2005, 28:314-317
Liu, Ranyi¡é ­é Huang, W. Construction of Adenovirus Harboring N-fragment of SARS-CoV, Journal of Sun Yat-sen University (Medical Sciences), 2005, 26:32-34
Wu, Jiang-xue, Huang, W. Research Advancement of Endogenous Angiogenesis Inhibitors, Chinese Journal of Cancer, 2005, 24(3):376-384
Liu, Ran-yi¡é ­é Huang, W. Adenoviral Expression of a Truncated S1 Subunit of SARS-CoV Spike Protein Results in Specific Humoral Immune Responses Against SARS-CoV in Rats, Virus Research, 2005:24-31
Tan,Wei-ping¡é ­é Huang,W. Construction of Murine T-bet cDNA Recombinant Adenovirus Vector and its Identification. Journal of Sun Yat-sen University (Medical Sciences), 2004,25:546-553
He, Gou-An¡é ­é .Huang, W. Dynamic Distribution and Expression in vivo of Human Endostatin Gene Deliveried by Adenoviral Vector, Life Science, 77:1331-1340,2005
Huang, Jialin¡é ­é .Huang, W. Th2 Predominance and CD8+ Memory T Cell Depletion in Patients with Severe Acute Respiratory Syndrome, Microbe and Infections, (7)427-436,2005
Li, Xiaoqing, Huang, Wenlin. In vitro Anti-leukeemic Effects of Bone Marrow Stromal Cells Modified with Ad/hIFN-r. Journal of Guangdong College of Pharmacy, 21:307-311, 2005
Huang,Jialing, Huang, Wenlin. Construction of Hepatocellular Carcinoma-Specific Eukarotypic Expression Vector Carrying Double Suicide Genes, Journal of Sun Yat-sen University (Medical Sciences),26(2):142-145, 2005
Wu, Jiangxue and Huang, Wenlin. Research Advancement of Endogenous Angiogenesis Inhibitors, Chinese Journal of Cancer, 24(3):376-384, 2005
Lili¡é ­é .Huang,W. Intra-tumoral Delivery of Recombinant Adenovirus Carrying Human Endostatin Gene Resulted in Inhibition of Angiogenesis in Nasopharyngeal Carcinom.Accepted by International Joural of Cancer,2005
Liang Zhihui¡é ­é Huang, W. Inhibition of Tumor Growth in Xenografted Mice with Adenovirus-mediated Endostatin Gene Comparision with Recombinant Endostatin Protein, Chinese Medical Journal (English Version), 117(12):1809-1814, 2004
Lili¡é ­é , Huang, W. Endostatin Gene Therapy for Liver Cancer by a Recombinant Adenovirus Delivery, World Journal of Gastrointestinal, 10(13):1867-1871, 2004
Duan, Zhaohui. Huang, Wenlin. Antibody Survey of SARS Patients, Chin J Lab Med, 27(12):836-837, 2004
Huang, W., S.J. Flint, Unusual Properties of Adenovirus E2E Transcription by RNA Polymerase III, Journal of Virology, 77, 4015-4024, 2003
Lili¡é ­é Huang,W. Inhibition of Hapatoma in Nude Mice by Recombinant Adenovirus Carrying Human Endostatin. Chinese Journal of Hepatology, 11(9):542-545,2003
Lili¡é ­é , Huang, W. Inhibition of Tumor Growth in Xenograft Nude Mice Model by Recombinant Adenovirus-mediated Human Endostatin Gene Therapy. Chin J Radiol.37(12):1068-1073, 2003
Ding,X¡é ­é Huang,W. Inhibition of Tongue Cancer Development in Nude Mice Transfected with Adenovirus Carrying Human Endostation Gene. Chinese Journal of Cancer, 22(11):1152-1157, 2003
Huang, W., etc. Inhibition of Adenovirus E1A Expression in HeLa Cells by Product R, a Peptide Nucleic Acid Immunomodulator. Journal of Infectious Diseases, 31-1, 328-231. 2001
Huang, W., etc. A Peptide Nucleic Acid Complex, Interferes with Expression of the E7 Oncoprotein of Human Papilloma Virus-18 in HeLa Cells. Journal of Infectious Diseases, 31-1, 220-227. 2000
An, H¡é ­é Huang, W., etc. Construction of p53 in Adenovirus Vector. Bulletin of Fourth Military Medical University,21(2):220-222, 2000
Huang, W., etc. Construction of Inducible Adenovirus Expression Vector, Journal of Medicine Chin. 12:73-80, 1998
Huang, W., Daiming Fang, Distribution of mRNA Expression of The Tyrosine Kinase C Receptor in Rat Spinal Cord? by in Situ Hybridization. Biochemistry, 10.30;(S:S):344, 1997
Liu, Y¡é ­é . Huang, W. etc., Application of Recombinant adenovirus for in vivo Gene Delivery to Spinal Cord. Brain Res, 768(1-2):19-29, 1997
Huang, W.; Flint, S.J. The Tripartite Leader Sequence of Subgroup C Adenovirus Major Late mRNA Can Increase the Efficiency of mRNA Export. Journal of Virology, 72, 225-235, 1998
Yang, U.C.; Huang, W.; Flint, S. J. Messenger RNA Export Correlates with Activation of Transcription in Human Subgroup C Adenovirus-Infected Cells. J. of Virology, 70, 4071-4080, 1996
Huang , W.; Pruzan, R.; Flint, S.J. In Vivo Transcription from the Adenovirus E2E Promoter by RNA Polymerase III. Proc. Natl. Acad. Sci., USA 91, 1265-1269, 1994
Pruzan, R; Huang, W.; Chatterjee, P.K.; Flint, S.J. RNA Polymerase II Transcription of the Adenovirus 2 E2E Promoter. DNA Virus Conference in Cold Spring Harbor Lab, Aug. 12-16, 1992
Li, X.; Huang, W.; Flint, S.J. The Downstream Regulatory Sequence of the Adenovirus Type 2 Major Late Promoter Is Functionally Reductant. Journal of Virology, 66, 5685-5690, 1992
Huang, W.; etc., A Study on Screening of Cancers by Using Extract of Human Placenta. Health Care Journal, 6, 147-148, 1990
Huang, W.; etc., Inhibition of Porcine Spleen Interferon to Human Hemorrhagic Virus on Tissue Culture. Journal of Biopharmacia, 3, 107-110, 1989
Huang, W.; etc., Serological Study on the RHDV. Virologica Sinica, 1 ,90-95, 1989
Huang, W.; etc., Antigenic Relationship Between Porcine Spleen Cell Interferon and Human Leukocyte Interferon. Virologica Sinica, 2, 193-198, 1989
Huang, W.; etc., Some Antigenic Relationship between Porcine Spleen IFN and HuIFN by McAb of HuIFN-alpha. Biochemistry and Biophysics Sinica 15, 231-233, 1988
Huang, W.; etc., Separation of the Porcine Spleen Interferon (PsIFN) on Fast Performance Liquid Chromatography (FPLC). Progress in Biochemistry and Biophysics, 15, 231-233, 1988
Huang, W.; etc., Research on Porcine Spleen Interferon. Second SCBA International Symposium at University of California, Berkeley, June 27, 1988
Huang, W.; etc., Approval on the Identification and Classification of Rabbit Hemorrhagic Disease Virus in Wuhan Virologica Sinica, 1, 7,1988
Huang, W.; etc., Study on the Heterogenic of Some Animal IFNs. Journal of Biochemical Pharmaceutic, 3, 43-49, 1987
Huang, W.; etc., Some Problems of Animal IFNs Heterogenic. Biochemistry of the Life, 7, 9-10,1987
Huang, W.; etc., Production and Antiviral Effects of a Porcine Leukocyte IFN. Acta Virologica Sinica, 5, 57-62,1987
Zhang, G., Huang, W. Serological Study on NPV Protein and Virion from the NPV of Cotton Bollom. Acta Microbiological Sinica, 25, 262-269,1985
Huang, W.; etc., Serological Detection of Some Animal Inoculated with Nuclear Polyhedron Virus (NPV) of Cotton Bollom. Acta Virological Sinica, 3, 159-163,1983
Sinica 1983,3:159-163